cultrex reduced growth factor basement membrane matrix Search Results


basement membrane extract bme  (R&D Systems)
96
R&D Systems basement membrane extract bme
Cytocompatibility of PFD. a) Exemplary image of MCF7 spheroids embedded in basement membrane extract <t>(BME)</t> with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. b) Exemplary image of <t>CRC</t> <t>organoids</t> embedded in BME with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. c) Cell viability 4 h after plating either via PFD or via manual pipetting (MP). Three technical replicates, n ≥ 60 for each sample type and each condition. d) Measured luminescence signal due to Annexin V binding to exposed phosphatidylserine on the cell surface as an indicator of apoptosis 24 h post plating. Three technical replicates, n ≥ 60 for each sample type and each condition. e) Measured loss of membrane integrity as indicator of secondary necrosis 24 h post plating. Three technical replicates, n≥60 for each sample type and each condition. f) Normalized area of unprocessed cell aggregates and aggregates that were processed with the spheroid and organoid processing platform. Each condition shows data of n ≥ 60. Aggregates with diameters larger than 240 µm were not considered for aspiration and are therefore excluded from the data set. g) Exemplary proliferation of an MCF7 spheroid that was automatically embedded in BME over the course of several days. Day 1 was the day of embedding. Scale bar: 100 µm. h) Fold change in diameter of MCF7 spheroids after they were embedded in basal membrane extract with the platform. n = 34. Spheroids were automatically delivered into the hydrogel on day 1. Data was tested for normal distribution and the two‐tailed t ‐test was performed. P‐ values ≥ 0.05 were considered nonsignificant (n.s.).
Basement Membrane Extract Bme, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+reduced+growth+factor+basement+membrane+matrix/Cultrex+Basement+Membrane+Extract%2C+Type+2/pmc11468932-291-11-25
Average 96 stars, based on 1 article reviews
basement membrane extract bme - by Bioz Stars, 2026-10
96/100 stars
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cultrex pathclear reduced growth factor bme  (Bio-Techne corporation)
96
Bio-Techne corporation cultrex pathclear reduced growth factor bme
Cytocompatibility of PFD. a) Exemplary image of MCF7 spheroids embedded in basement membrane extract <t>(BME)</t> with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. b) Exemplary image of <t>CRC</t> <t>organoids</t> embedded in BME with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. c) Cell viability 4 h after plating either via PFD or via manual pipetting (MP). Three technical replicates, n ≥ 60 for each sample type and each condition. d) Measured luminescence signal due to Annexin V binding to exposed phosphatidylserine on the cell surface as an indicator of apoptosis 24 h post plating. Three technical replicates, n ≥ 60 for each sample type and each condition. e) Measured loss of membrane integrity as indicator of secondary necrosis 24 h post plating. Three technical replicates, n≥60 for each sample type and each condition. f) Normalized area of unprocessed cell aggregates and aggregates that were processed with the spheroid and organoid processing platform. Each condition shows data of n ≥ 60. Aggregates with diameters larger than 240 µm were not considered for aspiration and are therefore excluded from the data set. g) Exemplary proliferation of an MCF7 spheroid that was automatically embedded in BME over the course of several days. Day 1 was the day of embedding. Scale bar: 100 µm. h) Fold change in diameter of MCF7 spheroids after they were embedded in basal membrane extract with the platform. n = 34. Spheroids were automatically delivered into the hydrogel on day 1. Data was tested for normal distribution and the two‐tailed t ‐test was performed. P‐ values ≥ 0.05 were considered nonsignificant (n.s.).
Cultrex Pathclear Reduced Growth Factor Bme, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+reduced+growth+factor+basement+membrane+matrix/Cultrex+RGF+Basement+Membrane+Extract%2C+Type+2/pm33879799-332-17-23
Average 96 stars, based on 1 article reviews
cultrex pathclear reduced growth factor bme - by Bioz Stars, 2026-10
96/100 stars
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matrigel growth factor reduced, phenol red-free, ldev-free  (Corning Life Sciences)
90
Corning Life Sciences matrigel growth factor reduced, phenol red-free, ldev-free
Cytocompatibility of PFD. a) Exemplary image of MCF7 spheroids embedded in basement membrane extract <t>(BME)</t> with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. b) Exemplary image of <t>CRC</t> <t>organoids</t> embedded in BME with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. c) Cell viability 4 h after plating either via PFD or via manual pipetting (MP). Three technical replicates, n ≥ 60 for each sample type and each condition. d) Measured luminescence signal due to Annexin V binding to exposed phosphatidylserine on the cell surface as an indicator of apoptosis 24 h post plating. Three technical replicates, n ≥ 60 for each sample type and each condition. e) Measured loss of membrane integrity as indicator of secondary necrosis 24 h post plating. Three technical replicates, n≥60 for each sample type and each condition. f) Normalized area of unprocessed cell aggregates and aggregates that were processed with the spheroid and organoid processing platform. Each condition shows data of n ≥ 60. Aggregates with diameters larger than 240 µm were not considered for aspiration and are therefore excluded from the data set. g) Exemplary proliferation of an MCF7 spheroid that was automatically embedded in BME over the course of several days. Day 1 was the day of embedding. Scale bar: 100 µm. h) Fold change in diameter of MCF7 spheroids after they were embedded in basal membrane extract with the platform. n = 34. Spheroids were automatically delivered into the hydrogel on day 1. Data was tested for normal distribution and the two‐tailed t ‐test was performed. P‐ values ≥ 0.05 were considered nonsignificant (n.s.).
Matrigel Growth Factor Reduced, Phenol Red Free, Ldev Free, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+reduced+growth+factor+basement+membrane+matrix/matrigel/pmc11579301-337-8-15
Average 90 stars, based on 1 article reviews
matrigel growth factor reduced, phenol red-free, ldev-free - by Bioz Stars, 2026-10
90/100 stars
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cultrex reduced growth factor bme  (R&D Systems)
97
R&D Systems cultrex reduced growth factor bme
Cytocompatibility of PFD. a) Exemplary image of MCF7 spheroids embedded in basement membrane extract <t>(BME)</t> with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. b) Exemplary image of <t>CRC</t> <t>organoids</t> embedded in BME with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. c) Cell viability 4 h after plating either via PFD or via manual pipetting (MP). Three technical replicates, n ≥ 60 for each sample type and each condition. d) Measured luminescence signal due to Annexin V binding to exposed phosphatidylserine on the cell surface as an indicator of apoptosis 24 h post plating. Three technical replicates, n ≥ 60 for each sample type and each condition. e) Measured loss of membrane integrity as indicator of secondary necrosis 24 h post plating. Three technical replicates, n≥60 for each sample type and each condition. f) Normalized area of unprocessed cell aggregates and aggregates that were processed with the spheroid and organoid processing platform. Each condition shows data of n ≥ 60. Aggregates with diameters larger than 240 µm were not considered for aspiration and are therefore excluded from the data set. g) Exemplary proliferation of an MCF7 spheroid that was automatically embedded in BME over the course of several days. Day 1 was the day of embedding. Scale bar: 100 µm. h) Fold change in diameter of MCF7 spheroids after they were embedded in basal membrane extract with the platform. n = 34. Spheroids were automatically delivered into the hydrogel on day 1. Data was tested for normal distribution and the two‐tailed t ‐test was performed. P‐ values ≥ 0.05 were considered nonsignificant (n.s.).
Cultrex Reduced Growth Factor Bme, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+reduced+growth+factor+basement+membrane+matrix/Cultrex+Reduced+Growth+Factor+Basement+Membrane+Extract%2C+Type+2%2C+Select/pmc09693993-166-11-16
Average 97 stars, based on 1 article reviews
cultrex reduced growth factor bme - by Bioz Stars, 2026-10
97/100 stars
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bme  (R&D Systems)
95
R&D Systems bme
Cytocompatibility of PFD. a) Exemplary image of MCF7 spheroids embedded in basement membrane extract <t>(BME)</t> with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. b) Exemplary image of <t>CRC</t> <t>organoids</t> embedded in BME with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. c) Cell viability 4 h after plating either via PFD or via manual pipetting (MP). Three technical replicates, n ≥ 60 for each sample type and each condition. d) Measured luminescence signal due to Annexin V binding to exposed phosphatidylserine on the cell surface as an indicator of apoptosis 24 h post plating. Three technical replicates, n ≥ 60 for each sample type and each condition. e) Measured loss of membrane integrity as indicator of secondary necrosis 24 h post plating. Three technical replicates, n≥60 for each sample type and each condition. f) Normalized area of unprocessed cell aggregates and aggregates that were processed with the spheroid and organoid processing platform. Each condition shows data of n ≥ 60. Aggregates with diameters larger than 240 µm were not considered for aspiration and are therefore excluded from the data set. g) Exemplary proliferation of an MCF7 spheroid that was automatically embedded in BME over the course of several days. Day 1 was the day of embedding. Scale bar: 100 µm. h) Fold change in diameter of MCF7 spheroids after they were embedded in basal membrane extract with the platform. n = 34. Spheroids were automatically delivered into the hydrogel on day 1. Data was tested for normal distribution and the two‐tailed t ‐test was performed. P‐ values ≥ 0.05 were considered nonsignificant (n.s.).
Bme, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+reduced+growth+factor+basement+membrane+matrix/Cultrex+UltiMatrix+RGF+Basement+Membrane+Extract/bio_rxiv__2025__07__15__662972-321-8-13
Average 95 stars, based on 1 article reviews
bme - by Bioz Stars, 2026-10
95/100 stars
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cultrex bme type 2  (R&D Systems)
96
R&D Systems cultrex bme type 2
Cytocompatibility of PFD. a) Exemplary image of MCF7 spheroids embedded in basement membrane extract <t>(BME)</t> with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. b) Exemplary image of <t>CRC</t> <t>organoids</t> embedded in BME with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. c) Cell viability 4 h after plating either via PFD or via manual pipetting (MP). Three technical replicates, n ≥ 60 for each sample type and each condition. d) Measured luminescence signal due to Annexin V binding to exposed phosphatidylserine on the cell surface as an indicator of apoptosis 24 h post plating. Three technical replicates, n ≥ 60 for each sample type and each condition. e) Measured loss of membrane integrity as indicator of secondary necrosis 24 h post plating. Three technical replicates, n≥60 for each sample type and each condition. f) Normalized area of unprocessed cell aggregates and aggregates that were processed with the spheroid and organoid processing platform. Each condition shows data of n ≥ 60. Aggregates with diameters larger than 240 µm were not considered for aspiration and are therefore excluded from the data set. g) Exemplary proliferation of an MCF7 spheroid that was automatically embedded in BME over the course of several days. Day 1 was the day of embedding. Scale bar: 100 µm. h) Fold change in diameter of MCF7 spheroids after they were embedded in basal membrane extract with the platform. n = 34. Spheroids were automatically delivered into the hydrogel on day 1. Data was tested for normal distribution and the two‐tailed t ‐test was performed. P‐ values ≥ 0.05 were considered nonsignificant (n.s.).
Cultrex Bme Type 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+reduced+growth+factor+basement+membrane+matrix/Cultrex+Reduced+Growth+Factor+Basement+Membrane+Extract%2C+Type+2%2C+Select/pm39453996-298-7-21
Average 96 stars, based on 1 article reviews
cultrex bme type 2 - by Bioz Stars, 2026-10
96/100 stars
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cultrex reduced growth factor basement membrane extract bme type 2  (R&D Systems)
96
R&D Systems cultrex reduced growth factor basement membrane extract bme type 2
Cytocompatibility of PFD. a) Exemplary image of MCF7 spheroids embedded in basement membrane extract <t>(BME)</t> with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. b) Exemplary image of <t>CRC</t> <t>organoids</t> embedded in BME with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. c) Cell viability 4 h after plating either via PFD or via manual pipetting (MP). Three technical replicates, n ≥ 60 for each sample type and each condition. d) Measured luminescence signal due to Annexin V binding to exposed phosphatidylserine on the cell surface as an indicator of apoptosis 24 h post plating. Three technical replicates, n ≥ 60 for each sample type and each condition. e) Measured loss of membrane integrity as indicator of secondary necrosis 24 h post plating. Three technical replicates, n≥60 for each sample type and each condition. f) Normalized area of unprocessed cell aggregates and aggregates that were processed with the spheroid and organoid processing platform. Each condition shows data of n ≥ 60. Aggregates with diameters larger than 240 µm were not considered for aspiration and are therefore excluded from the data set. g) Exemplary proliferation of an MCF7 spheroid that was automatically embedded in BME over the course of several days. Day 1 was the day of embedding. Scale bar: 100 µm. h) Fold change in diameter of MCF7 spheroids after they were embedded in basal membrane extract with the platform. n = 34. Spheroids were automatically delivered into the hydrogel on day 1. Data was tested for normal distribution and the two‐tailed t ‐test was performed. P‐ values ≥ 0.05 were considered nonsignificant (n.s.).
Cultrex Reduced Growth Factor Basement Membrane Extract Bme Type 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+reduced+growth+factor+basement+membrane+matrix/Cultrex+RGF+Basement+Membrane+Extract%2C+Type+2/bio_rxiv__2025__11__28__691167-293-9-19
Average 96 stars, based on 1 article reviews
cultrex reduced growth factor basement membrane extract bme type 2 - by Bioz Stars, 2026-10
96/100 stars
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cultrex 3d culture matrix reduced growth factor basement membrane  (Fisher Scientific)
86
Fisher Scientific cultrex 3d culture matrix reduced growth factor basement membrane
Cytocompatibility of PFD. a) Exemplary image of MCF7 spheroids embedded in basement membrane extract <t>(BME)</t> with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. b) Exemplary image of <t>CRC</t> <t>organoids</t> embedded in BME with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. c) Cell viability 4 h after plating either via PFD or via manual pipetting (MP). Three technical replicates, n ≥ 60 for each sample type and each condition. d) Measured luminescence signal due to Annexin V binding to exposed phosphatidylserine on the cell surface as an indicator of apoptosis 24 h post plating. Three technical replicates, n ≥ 60 for each sample type and each condition. e) Measured loss of membrane integrity as indicator of secondary necrosis 24 h post plating. Three technical replicates, n≥60 for each sample type and each condition. f) Normalized area of unprocessed cell aggregates and aggregates that were processed with the spheroid and organoid processing platform. Each condition shows data of n ≥ 60. Aggregates with diameters larger than 240 µm were not considered for aspiration and are therefore excluded from the data set. g) Exemplary proliferation of an MCF7 spheroid that was automatically embedded in BME over the course of several days. Day 1 was the day of embedding. Scale bar: 100 µm. h) Fold change in diameter of MCF7 spheroids after they were embedded in basal membrane extract with the platform. n = 34. Spheroids were automatically delivered into the hydrogel on day 1. Data was tested for normal distribution and the two‐tailed t ‐test was performed. P‐ values ≥ 0.05 were considered nonsignificant (n.s.).
Cultrex 3d Culture Matrix Reduced Growth Factor Basement Membrane, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+reduced+growth+factor+basement+membrane+matrix/factor+growth+matrigel+reduced/pm40866413-503-7-16
Average 86 stars, based on 1 article reviews
cultrex 3d culture matrix reduced growth factor basement membrane - by Bioz Stars, 2026-10
86/100 stars
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type 2  (R&D Systems)
94
R&D Systems type 2
Cytocompatibility of PFD. a) Exemplary image of MCF7 spheroids embedded in basement membrane extract <t>(BME)</t> with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. b) Exemplary image of <t>CRC</t> <t>organoids</t> embedded in BME with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. c) Cell viability 4 h after plating either via PFD or via manual pipetting (MP). Three technical replicates, n ≥ 60 for each sample type and each condition. d) Measured luminescence signal due to Annexin V binding to exposed phosphatidylserine on the cell surface as an indicator of apoptosis 24 h post plating. Three technical replicates, n ≥ 60 for each sample type and each condition. e) Measured loss of membrane integrity as indicator of secondary necrosis 24 h post plating. Three technical replicates, n≥60 for each sample type and each condition. f) Normalized area of unprocessed cell aggregates and aggregates that were processed with the spheroid and organoid processing platform. Each condition shows data of n ≥ 60. Aggregates with diameters larger than 240 µm were not considered for aspiration and are therefore excluded from the data set. g) Exemplary proliferation of an MCF7 spheroid that was automatically embedded in BME over the course of several days. Day 1 was the day of embedding. Scale bar: 100 µm. h) Fold change in diameter of MCF7 spheroids after they were embedded in basal membrane extract with the platform. n = 34. Spheroids were automatically delivered into the hydrogel on day 1. Data was tested for normal distribution and the two‐tailed t ‐test was performed. P‐ values ≥ 0.05 were considered nonsignificant (n.s.).
Type 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+reduced+growth+factor+basement+membrane+matrix/Cultrex+RGF+Basement+Membrane+Extract%2C+Type+2/pm33504990-110-214-216
Average 94 stars, based on 1 article reviews
type 2 - by Bioz Stars, 2026-10
94/100 stars
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cultrex rgf bme type 2 gel  (R&D Systems)
95
R&D Systems cultrex rgf bme type 2 gel
Cytocompatibility of PFD. a) Exemplary image of MCF7 spheroids embedded in basement membrane extract <t>(BME)</t> with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. b) Exemplary image of <t>CRC</t> <t>organoids</t> embedded in BME with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. c) Cell viability 4 h after plating either via PFD or via manual pipetting (MP). Three technical replicates, n ≥ 60 for each sample type and each condition. d) Measured luminescence signal due to Annexin V binding to exposed phosphatidylserine on the cell surface as an indicator of apoptosis 24 h post plating. Three technical replicates, n ≥ 60 for each sample type and each condition. e) Measured loss of membrane integrity as indicator of secondary necrosis 24 h post plating. Three technical replicates, n≥60 for each sample type and each condition. f) Normalized area of unprocessed cell aggregates and aggregates that were processed with the spheroid and organoid processing platform. Each condition shows data of n ≥ 60. Aggregates with diameters larger than 240 µm were not considered for aspiration and are therefore excluded from the data set. g) Exemplary proliferation of an MCF7 spheroid that was automatically embedded in BME over the course of several days. Day 1 was the day of embedding. Scale bar: 100 µm. h) Fold change in diameter of MCF7 spheroids after they were embedded in basal membrane extract with the platform. n = 34. Spheroids were automatically delivered into the hydrogel on day 1. Data was tested for normal distribution and the two‐tailed t ‐test was performed. P‐ values ≥ 0.05 were considered nonsignificant (n.s.).
Cultrex Rgf Bme Type 2 Gel, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+reduced+growth+factor+basement+membrane+matrix/Cultrex+RGF+Basement+Membrane+Extract%2C+Type+2/pm38982588-96-8-14
Average 95 stars, based on 1 article reviews
cultrex rgf bme type 2 gel - by Bioz Stars, 2026-10
95/100 stars
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bme cultrex reduced growth factor basement membrane extract  (R&D Systems)
94
R&D Systems bme cultrex reduced growth factor basement membrane extract
Cytocompatibility of PFD. a) Exemplary image of MCF7 spheroids embedded in basement membrane extract <t>(BME)</t> with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. b) Exemplary image of <t>CRC</t> <t>organoids</t> embedded in BME with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. c) Cell viability 4 h after plating either via PFD or via manual pipetting (MP). Three technical replicates, n ≥ 60 for each sample type and each condition. d) Measured luminescence signal due to Annexin V binding to exposed phosphatidylserine on the cell surface as an indicator of apoptosis 24 h post plating. Three technical replicates, n ≥ 60 for each sample type and each condition. e) Measured loss of membrane integrity as indicator of secondary necrosis 24 h post plating. Three technical replicates, n≥60 for each sample type and each condition. f) Normalized area of unprocessed cell aggregates and aggregates that were processed with the spheroid and organoid processing platform. Each condition shows data of n ≥ 60. Aggregates with diameters larger than 240 µm were not considered for aspiration and are therefore excluded from the data set. g) Exemplary proliferation of an MCF7 spheroid that was automatically embedded in BME over the course of several days. Day 1 was the day of embedding. Scale bar: 100 µm. h) Fold change in diameter of MCF7 spheroids after they were embedded in basal membrane extract with the platform. n = 34. Spheroids were automatically delivered into the hydrogel on day 1. Data was tested for normal distribution and the two‐tailed t ‐test was performed. P‐ values ≥ 0.05 were considered nonsignificant (n.s.).
Bme Cultrex Reduced Growth Factor Basement Membrane Extract, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+reduced+growth+factor+basement+membrane+matrix/Cultrex+RGF+Basement+Membrane+Extract/pm39638255-55-8-18
Average 94 stars, based on 1 article reviews
bme cultrex reduced growth factor basement membrane extract - by Bioz Stars, 2026-10
94/100 stars
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cultrex reduced growth factor basement membrane extract r1 bme r1  (R&D Systems)
94
R&D Systems cultrex reduced growth factor basement membrane extract r1 bme r1
Cytocompatibility of PFD. a) Exemplary image of MCF7 spheroids embedded in basement membrane extract <t>(BME)</t> with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. b) Exemplary image of <t>CRC</t> <t>organoids</t> embedded in BME with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. c) Cell viability 4 h after plating either via PFD or via manual pipetting (MP). Three technical replicates, n ≥ 60 for each sample type and each condition. d) Measured luminescence signal due to Annexin V binding to exposed phosphatidylserine on the cell surface as an indicator of apoptosis 24 h post plating. Three technical replicates, n ≥ 60 for each sample type and each condition. e) Measured loss of membrane integrity as indicator of secondary necrosis 24 h post plating. Three technical replicates, n≥60 for each sample type and each condition. f) Normalized area of unprocessed cell aggregates and aggregates that were processed with the spheroid and organoid processing platform. Each condition shows data of n ≥ 60. Aggregates with diameters larger than 240 µm were not considered for aspiration and are therefore excluded from the data set. g) Exemplary proliferation of an MCF7 spheroid that was automatically embedded in BME over the course of several days. Day 1 was the day of embedding. Scale bar: 100 µm. h) Fold change in diameter of MCF7 spheroids after they were embedded in basal membrane extract with the platform. n = 34. Spheroids were automatically delivered into the hydrogel on day 1. Data was tested for normal distribution and the two‐tailed t ‐test was performed. P‐ values ≥ 0.05 were considered nonsignificant (n.s.).
Cultrex Reduced Growth Factor Basement Membrane Extract R1 Bme R1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cultrex+reduced+growth+factor+basement+membrane+matrix/Cultrex+RGF+Basement+Membrane+Extract%2C+Type+R1/pmc11849689-122-20-29
Average 94 stars, based on 1 article reviews
cultrex reduced growth factor basement membrane extract r1 bme r1 - by Bioz Stars, 2026-10
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Image Search Results


Cytocompatibility of PFD. a) Exemplary image of MCF7 spheroids embedded in basement membrane extract (BME) with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. b) Exemplary image of CRC organoids embedded in BME with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. c) Cell viability 4 h after plating either via PFD or via manual pipetting (MP). Three technical replicates, n ≥ 60 for each sample type and each condition. d) Measured luminescence signal due to Annexin V binding to exposed phosphatidylserine on the cell surface as an indicator of apoptosis 24 h post plating. Three technical replicates, n ≥ 60 for each sample type and each condition. e) Measured loss of membrane integrity as indicator of secondary necrosis 24 h post plating. Three technical replicates, n≥60 for each sample type and each condition. f) Normalized area of unprocessed cell aggregates and aggregates that were processed with the spheroid and organoid processing platform. Each condition shows data of n ≥ 60. Aggregates with diameters larger than 240 µm were not considered for aspiration and are therefore excluded from the data set. g) Exemplary proliferation of an MCF7 spheroid that was automatically embedded in BME over the course of several days. Day 1 was the day of embedding. Scale bar: 100 µm. h) Fold change in diameter of MCF7 spheroids after they were embedded in basal membrane extract with the platform. n = 34. Spheroids were automatically delivered into the hydrogel on day 1. Data was tested for normal distribution and the two‐tailed t ‐test was performed. P‐ values ≥ 0.05 were considered nonsignificant (n.s.).

Journal: Advanced Healthcare Materials

Article Title: Towards Automation in 3D Cell Culture: Selective and Gentle High‐Throughput Handling of Spheroids and Organoids via Novel Pick‐Flow‐Drop Principle

doi: 10.1002/adhm.202303350

Figure Lengend Snippet: Cytocompatibility of PFD. a) Exemplary image of MCF7 spheroids embedded in basement membrane extract (BME) with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. b) Exemplary image of CRC organoids embedded in BME with PFD and by manual pipetting one hour after plating. Scale bar: 200 µm. c) Cell viability 4 h after plating either via PFD or via manual pipetting (MP). Three technical replicates, n ≥ 60 for each sample type and each condition. d) Measured luminescence signal due to Annexin V binding to exposed phosphatidylserine on the cell surface as an indicator of apoptosis 24 h post plating. Three technical replicates, n ≥ 60 for each sample type and each condition. e) Measured loss of membrane integrity as indicator of secondary necrosis 24 h post plating. Three technical replicates, n≥60 for each sample type and each condition. f) Normalized area of unprocessed cell aggregates and aggregates that were processed with the spheroid and organoid processing platform. Each condition shows data of n ≥ 60. Aggregates with diameters larger than 240 µm were not considered for aspiration and are therefore excluded from the data set. g) Exemplary proliferation of an MCF7 spheroid that was automatically embedded in BME over the course of several days. Day 1 was the day of embedding. Scale bar: 100 µm. h) Fold change in diameter of MCF7 spheroids after they were embedded in basal membrane extract with the platform. n = 34. Spheroids were automatically delivered into the hydrogel on day 1. Data was tested for normal distribution and the two‐tailed t ‐test was performed. P‐ values ≥ 0.05 were considered nonsignificant (n.s.).

Article Snippet: Briefly, organoids were cultivated in domes of 4 mg mL −1 basement membrane extract BME (Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear, R&D Systems, Inc., USA).

Techniques: Membrane, Binding Assay, Two Tailed Test